The 2023 Multi-Sector Needs Assessment (MSNA) in Czechia presents findings from a regional interagency multi-sectoral assessment conducted with Ukrainian refugees in Czechia from June to July 2023. The assessment aimed to capture the needs of and access to basic services for refugees, identify service gaps, and understand refugees' priorities for the coming year to inform response planning. Using a structured questionnaire, the assessment surveyed purposively selected households of refugees from Ukraine, stratified by geographic area. Key topics included health, protection, food security, education, livelihoods, gender-based violence, mental health, cash assistance, and legal needs. As a key source of information for response planning, the 2023 MSNA provides insights into the experiences and priority needs of Ukrainian refugees in Czechia.